Magilton's side had the weekend off which should have allowed midfielder Alan Quinn to further recover from his ankle injury.
The former Sheffield United man has been nursing an ankle injury but made a 20-minute substitute appearance in the 1-1 draw with his former club last Tuesday and should again be in the squad.
Full-back Luis Castro Sito has turned his ankle in the reserves and will be out although he has not been involved since his red card at Blackpool in January anyway.
Forward Jon Walters is nursing a knee injury which often causes him to miss training but he should again play through the discomfort.
Walters moved back out to the right wing against the Blades after a stint up front to allow Pablo Counago to return. Magilton has a wealth of midfield options and Owen Garvan, Quinn and Tommy Miller are all hopeful of a return after being on the bench last time out.
Marciano Van Homoet, Martin Devaney and Dennis Souza are all doubtful for Barnsley.
Dutch left-back Van Homoet is struggling with an ankle injury, winger Martin Devaney has a broken nose and Brazilian defender Souza has a tight hamstring.
All three players sustained their respective injuries in the Tykes' FA Cup win over Chelsea on Saturday and manager Simon Davey will monitor their progress.
Lewin Nyatanga, cup-tied for the FA Cup quarter-final, could step in if Souza fails to make it, while Sam Togwell is a likely replacement for Devaney, who he replaced in the second half on Saturday.
Davey could be tempted to make further changes after revealing some of his players were mentally and physically jaded following the Tykes' cup heroics.
The Barnsley boss is anxious his side secure their first league win in six matches as they are currently just four points clear of the drop zone.
Sporting Life