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"It's been bothering me a little over the last game or two and I felt it stiffening up, so there was no option but to come off.
"It eased after I had a shower and now it's a case of waiting to see what the scan shows up."
Dave Williams the Town Physio added: "There is no point in guessing what the damage is at this stage.
"We'll have a look at the scans and go from there, but obviously the fact that Alan has come off makes him doubtful for the weekend."
Another doubt for Saturdays match is defender Fabian Wilnis who was substituted just after half time suffering from a rib injury.
Fabian told the club website: "I was really struggling to breathe and it just got to the point where I couldn't carry on.
"The way I feel at the moment I can't see myself being fit for the Wolves game at the weekend, but hopefully it will ease quickly.
"I'm just hoping it's not what Jason had at the start of the season."
De Vos missed the first month of the season after breaking a rib in the opening day victory over Cardiff.